Fig. 2

Inhibiting mitochondrial fission with Mdivi-1 reduces morphine-induced behavior and mitochondria morphological damage. (A-B) Experimental scheme of the behavioral tests for saline and morphine groups treated with vehicle or Mdivi-1. (C) The preference of the CPP experimental mice during pre-test and post-test. (n = 11 mice in each group). (D) Quantification of the morphine CPP score in the mice injected with Mdivi-1 or vehicle. (n = 11 mice in each group). (E-I) qRT-PCR analysis of TNFα, IL-1β, Drp1, Fis1 and Mff expression in NAc (n = 4 mice in each group). (J) Representative electron microscope images of the mitochondria morphology in NAc neurons, astrocytes and microglia from treated mice (scale bar, 500 nm). (K-M) Cumulative frequency distribution graph and quantification of mitochondrial length in NAc neurons (K), astrocytes (L) and microglia (M) (n = 4 mice in each group). Data are presented as mean ± SEM; C, D: p-values were calculated by two-way ANOVA with Sidak’s multiple comparison. E-I, K-M: p-values were calculated by one-way ANOVA with Sidak’s multiple comparison. ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001