Fig. 2

Single-cell profiling of the meningeal microenvironment after SAH. A, B UMAP projection of cells in the meninges colored by manually annotated clusters (A) and samples (B). C Stacked bar plot showing the proportion of cell clusters in each group. D Dot plot illustrating the expression levels of cell typing genes in each cluster using Wang et al. as a reference [2]. E KEGG enrichment analyses of ranked gene lists from three different sample groups based on GSEA. F Flow cytometric analysis of myeloid-to-lymphocyte ratio of CD45 high cells in the SAH 24 h and SAH 72 h group G Flow cytometric analysis of neutrophil and mono-macrophage percentage of CD45 high cells in the SAH 24 h and SAH 72 h group. H Flow cytometric analysis of T, NK and B cell percentage of CD45 high cells in the SAH 24 h and SAH 72 h group. (n = 10, the dura mater from two mice in the same group were combined into one sample for flow cytometry; unpaired two-tailed Mann–Whitney test for B cell percentage, unpaired two-tailed Student’s t-test for others). All data was represented as mean ± SD. *p < 0.05, **p < 0.01, ns represents no statistical difference. I The spatial spot map corresponding to the annotations of single-cell sequence, with different colored spots representing different clusters