Fig. 2
From: Astrocytic lipid droplets contain MHCII and may act as cogs in the antigen presentation machinery
MHCII is stored within astrocytic LDs. ICC of PLIN3 in combination with LipidTOX, show distinct PLIN3-coated LDs in hiPSC-derived astrocytes (a). ImmunoTEM of isolated LD verifying the presence of PLIN3 (arrows) on the surface of LDs (b). In some astrocytes, MHCII is situated within PLIN3 coated droplets (c). LipidTOX staining confirming MHCII co-localization with LDs (d). Exposure to OA resulted in a significant increase of both the percentage MHCII + LDs (e) and total MHCII levels (f), compared to control astrocytes. The percentage of MHCII + LDs, showed a significant decrease in Aβ exposed astrocytes compared to control astrocytes (g). For the statistical analysis of e-g the N number is 3 (shown in the graphs with different symbols and colors). Data is shown as scatterplots with mean and standard deviation. The level of significance for all the graphs is: * = P < 0.05, ** = P < 0.01, and *** = P < 0.001. LDs and MHCII are not situated in LAMP1 + endosomal/lysosomal vesicles (h). CD74 co-localizes with cytoplasmic MHCII (i), but not with MHCII situated in LDs (j). MHCII signal detected with the active conformation specific clone L432 within PLIN3 coated droplets (k). Scale bar (a-c, h-j, k): 20 μm; (d): 2 μm; (zoomed in i-j, k’): 10 μm