Fig. 7

Inflammatory cytokines at the lesion of lumbar spinal cord during EAE. RT-qPCR analysis showing the higher levels of TNFα and IFNγ in the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice compared to WT controls at PID-14 (A) but comparable at PID-30 (B). No significant differences of TNFα and IFNγ levels were seen between the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice (A, B). No significant differences of IL-17 and IL-10 levels were seen among the lumbar spinal cord of WT, f-ATXN1^146Q/2Q and Atxn1^2Q/− mice at PID-14 (A) and PID-30 (B). At PID-30, Atxn1^2Q/− mice showed lower levels of iNOS compared to WT controls, whereas no significant differences were observed among all three groups of mice at PID-14 (A, B). N = 5 animals each. Statistical analyses were done with one-way ANOVA with Tukey’s multiple comparison test. Error bars represent SEM; *p ≤ 0.05, ns, not significant