Fig. 6

Infiltration of inflammatory T cells and macrophages/microglia in the white matter of EAE-induced lumbar spinal cord lesions at early and late Post Immunization Days. (A–F, M) DAB staining of CD3 (T cells) showed higher number of T cells at PID-14 (A–C) as well as at PID-30 (D–F) in the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice compared to WT controls. No significant differences were seen between the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice. (G–J, N) DAB staining of Iba1 (macrophages/microglia) showed a higher number of macrophages/microglia at PID-14 (G–I) as well as at PID-30 (J–L) in the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice compared to WT controls. No significant differences were seen between the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice. (G–J, O) Morphology analysis showed a higher number of hypertrophic and/or amoeboid macrophages/microglia at PID-14 as well as at PID-30 in the lumbar spinal cord of f-ATXN1^146Q/2Q and Atxn1^2Q/− mice compared to WT controls (O). A significantly higher number of hypertrophic and/or amoeboid macrophages/microglia were observed at PID-14, but their numbers were comparable between the f-ATXN1^146Q/2Q and Atxn1^2Q/− mice at PID-30 (O). N = 4 animals each. Scale bars: 40 μm. Black arrows indicate single cells, and red arrows indicate clusters of activated T cells and macrophages/microglia. Statistical analyses were done with one-way ANOVA with Tukey’s multiple comparison test. Error bars represent SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ns, not significant