Fig. 5
Alteration in EAE-induced reactive astrocytes at early and late Post Immunization Days. GFAP immunofluorescence staining (green) and nuclear counterstain, DAPI (blue) showed ablation of reactive astrocytes in the white matter of lumbar spinal cord of f-ATXN1146Q/2Qand Atxn12Q/− mice compare to WT controls in the acute phage of disease progression at PID-14 (A–C, G). In the chronic phage of diseases progression at PID-30 (D–f, G), the elevated number of reactive astrocytes were counted in the white matter of lumbar spinal cord of both f-ATXN1146Q/2Q and Atxn12Q/− mice compared to WT controls. N = 4 animals each. Scale bars: 40 μm. RT-qPCR analysis showing the lower levels of GFAP and higher levels of C3 in the lumbar spinal cord of f-ATXN1146Q/2Q and Atxn12Q/− mice compared to WT controls at PID-14 (H), while levels were comparable among three groups at PID-30 (I). No significant differences of Clcf1 and Slc1a2 expressions were observed in the lumbar spinal cord of WT, f-ATXN1146Q/2Q, and Atxn12Q/− mice at either PID-14 (H) or PID-30 (I). N = 4–5 animals each. Statistical analyses were done with one-way ANOVA with Tukey’s multiple comparison test. Error bars represent SEM; *p ≤ 0.05, **p ≤ 0.01, ns, not significant