Fig. 4

EAE-induced axon degeneration is enhanced in f-ATXN1^146Q/2Q and Atxn1^2Q/− mice. (A–C, J) Immunofluorescence staining of the NF-H for degenerating axons in the lumbar spinal cord showed that no axon degeneration was found in Naïve mice at age of 8 weeks (A–C). (D–F, J) Immunofluorescence staining of NF-H in lumbar spinal cord displayed significantly higher number of degenerating axons in f-ATXN1^146Q/2Q and Atxn1^2Q/− mice during accelerated disease progression at PID-14 (D–F) and during remission at PID-30 (G–I) compared to WT mice. (J) f-ATXN1^146Q/2Q and Atxn1^2Q/− mice showed comparable number of degenerating axons at both time points. N = 4 animals each. Scale bars: 40 μm. Statistical analyses were done with one-way ANOVA with Tukey’s multiple comparison test. Error bars represent SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.001, ns, not significant