Fig. 3

EAE-induced oligodendrocyte loss and demyelination are more severe in f-ATXN1^146Q/2Q and Atxn1^2Q/− mice. (A) Diagram depicting the sample collection protocol. (B–G, N) DAB staining of the CC1 in the lumbar spinal cord showed significantly higher loss of oligodendrocytes in f-ATXN1^146Q/2Q and Atxn1^2Q/− mice during peak disease at PID-14 (B–D) and during remission at PID-30 (E–G), as with WT mice. There were no differences in the level of oligodendrocyte loss between f-ATXN1^146Q/2Q mice and Atxn1^2Q/− mice at PID-14 and PID-30. (H–M, O) DAB staining of the MBP showed that the percentage of demyelinated area in the lumbar spinal cord was significantly increased in f-ATXN1^146Q/2Q mice and Atxn1^2Q/− mice compared to WT mice at PID-14 (H–J) and PID-30 (K–M). No significant differences in the degree of demyelination in the lumbar spinal cord were observed between f-ATXN1^146Q/2Q and Atxn1^2Q/− mice at PID-14 and PID-30. N = 4 animals each. Scale bars: 40 μm (B–G) and 100 μm (H–M). Statistical analyses were done with one-way ANOVA with Tukey’s multiple comparison test. Error bars represent SEM; *p ≤ 0.05, **p ≤ 0.01, ns, not significant