Fig. 6

Impaired microglial phagocytosis was accompanied by downregulation of Mas1 in vivo (A) Bar graph of the average expression levels of G protein-coupled receptor family members at 3 dpi and 7 dpi in macrophages/microglia after SCI transcriptomic data (GSE113566). (B) Representative immunofluorescence images of Cx3cr1⁺ microglia (red), Mas1 (green) at 0–7 dpi in WT mice. The lesion core is marked with asterisks (*). (C) Western blot analysis of Mas1 at 0–7 dpi in WT mice. (D) Quantification of Cx3cr1⁺MBP⁺ microglia numbers at 0–7 dpi in WT mice. Hollow data points represent values from male mice. Solid data points denote female mice. (E) Representative immunofluorescence images of Mas1 in primary microglia incubated with myelin (1 mg/ml) at 0–5 d. (F) Western blot analysis of Mas1 in primary microglia incubated with myelin (1 mg/ml) at 0–5 d. (G) Quantification of Mas1 in primary microglia incubated with myelin (1 mg/ml) at 0–5 d (relative to GAPDH expression) in (C). (H) Quantification of Mas1 in primary microglia incubated with myelin (1 mg/ml) at 0–5 d (relative to GAPDH expression) in (F). (I) Quantification of MFI for Mas1 in primary microglia incubated with myelin (1 mg/ml) at 0–5 d in (E). Scale bar: 50 μm (B) and 20 μm (E). Data are presented as means ± SD. n = 7 mice per group in (D). n = 3 independent cultures in (G-I). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 by oneway ANOVA followed by Tukey’s post hoc test