Fig. 4

The change trend of Fascin-1 phosphorylation during microglial phagocytosis in vitro. (A) Representative immunofluorescence images of PKCα (red) and PKCγ (green) in primary microglia incubated with myelin (1 mg/ml) at 0–5 d. (B) Quantification of MFI for Fascin-1, p-Fascin-1, PKCα and PKCγ at 0–5 d. (C) Representative immunofluorescence images of p-Fascin-1 (red) and Fascin-1 (green) in primary microglia incubated with myelin (1 mg/ml) at 0–5 d. (D) Quantification of p-Fascin-1/Fascin-1 in (C). (E) Western blot analysis of Fascin-1, p-Fascin-1, PKCα and PKCγ in primary microglia incubated with myelin (1 mg/ml) at 0–5 d. (F) Quantification of Fascin-1, p-Fascin-1, PKCα and PKCγ at 0–5 d (relative to GAPDH expression) in (E). (G) Quantification of p-Fascin-1/Fascin-1 in (E). Scale bars: 20 μm (A) and (C). Data are presented as means ± SD. n = 3 independent cultures in (B), (D), (F), and (G). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 by oneway ANOVA followed by Tukey’s post hoc test