Fig. 3

Impaired microglial phagocytosis is accompanied by downregulation of Fascin-1 phosphorylation in vivo (A) Representative immunofluorescence images of p-Fascin-1 (red) and Fascin-1 (green) at 1, 3, and 7 dpi in WT mice. (B) Quantification of Mean Fluorescent Intensity (MFI) for p-Fascin-1 and Fascin-1 at 1, 3, and 7 dpi in WT mice. Hollow data points represent values from male mice. Solid data points denote female mice. (C) Quantification of p-Fascin-1/Fascin-1 in (A). (D) Gene ontology (GO) analysis of the published transcriptomic data (GSE113566) depicts the top 10 functional categories of differentially expressed genes in macrophages/microglia after SCI. (E) Bar graph of the average expression levels of PKC family members at 0–14 dpi in macrophages/microglia. (F) Western blot analysis of Fascin-1, p-Fascin-1, PKCα and PKCγ at 1, 3, and 7 dpi in WT mice. (G) Quantification of Fascin-1, p-Fascin-1, PKCα and PKCγ at 1, 3, and 7 dpi in WT mice (relative to GAPDH expression) in (F). (H) Quantification of p-Fascin-1/Fascin-1 in (F). Scale bar: 10 μm (A). Data are presented as means ± SD. n = 7 mice per group in (B) and (C). n = 3 mice per group in (G) and (H). ^*p < 0.05, ^**p < 0.01 by oneway ANOVA followed by Tukey’s post hoc test