Fig. 2

Fascin-1 regulates microglial phagocytosis in vivo and vitro (A) Representative immunofluorescence images of Cx3cr1⁺ microglia (red) and MBP (green) at 1, 3 and 7 dpi in Fascin-1^fl/fl and Fascin-1 CKO mice. White arrowheads indicate microglia phagocytosing MBP. (B) Quantification of the percentage of Cx3cr1⁺ cells containing MBP in (A). Hollow data points represent values from male mice. Solid data points denote female mice. (C) Representative immunofluorescence images of primary microglia (phalloidin staining, red) incubated with Dio-myelin (1 mg/ml, green) at 0–5 d in vitro. (D) Quantification of intensity of Dio-Myelin staining/cell in (C). Scale bars: 5 μm (A) and 20 μm (C). Data are presented as means ± SD. n = 7 mice per group in (B). n = 3 independent cultures in (D). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 by twoway ANOVA followed by Tukey’s post hoc test