Fig. 7

COX-2 inhibition alleviates neuroinflammation and oxidative stress in the BAVM pathological microenvironment. A Representative fluorescence images of immunostaining with CD31 and eNOS. Scale bar = 20 μm. The asterisk (*) represents the vascular lumen of BAVM. B Representative western blot images of eNOS in brain tissue. C The quantification of eNOS/β-actin (n = 4 mice). D, E Relative mRNA levels of Nos3 and Vegf-a in each group (n = 5). F The quantification of the Evans Blue Dye fluorescence area of each group (n = 6 mice). G Representative images of Evans Blue Dye perfusion. Scale bar = 20 μm. The asterisk (*) represents the vascular lumen of BAVM. H Representative fluorescence images of immunostaining with CD31 and OCCLUDIN (OCLN) for each group. Scale bar = 20 μm. The asterisk (*) represents the vascular lumen of BAVM. I The quantification of the ratio of OCLN + vessels/CD31 + vessels (%) (n = 6 mice). J Representative fluorescence images of immunostaining with CD31 and CLAUDIN-5 (CLDN5) for each group. Scale bar = 20 μm. The asterisk (*) represents the vascular lumen of BAVM. K The quantification of the ratio of CLDN5 + vessels/CD31 + vessels (%) (n = 6 mice)