Fig. 2

A distinct cell cluster termed injury-associated (IC) cells revealed by snRNAseq and immunohistochemistry. (A) Representative histological overview of brain sections stained with (from left to right) Gat/vGlut, IBA1/GFAP, CD68/IBA1, CD31/CD13, co-stained with DAPI. (B) Quantification of NeuN+, GFAP+, IBA1+, CD68+, CD31 + and CD13 + expression relative to intact tissue (dotted line). (C) Representative histological overview of brain sections stained with PDGFRb and co-stained with DAPI. (D) Quantification of PDGFRb + expression relative to intact tissue (dotted line). (E) Representative histological overview of brain sections stained with ITGAV and co-stained with DAPI. (F) Quantification of ITGAV + expression relative to intact tissue (dotted line). (G) Bar graph showing relative amount of major GABA and glutamatergic neuronal subtypes. (H) Dot plot showing expression of canonical cell type marker of injury-associated cells (IC), FB, and Asc. (I) Heatmap showing correlation of gene expression profiles between each cell type from stroke tissue. (J) Representative histological overview of brain sections stained with IGFBP5 (left) and quantification of IGFBP5 expression relative to intact tissue (right). (K) Feature plot showing the expression patterns of Apoe, Adam12, Cola1a2, and Vim in cells from stroke tissue. (L) Representative histological overview of brain sections stained with (from left to right) IGFBP5/GFAP and IGFBP/PDGFRB, co-stained with DAPI. (M) Cell counts (GFAP + and PDGFRb + positive cells, relative to all counted IGFBP5-positive cells) in the ibz and the stroke area. The data was generated with a cohort of n = 9 mice