Fig. 7
From: Single cell RNA sequencing after moderate traumatic brain injury: effects of therapeutic hypothermia
Validation of Bdnf expression using fluorescent in situ hybridization. (A) Dot plot of Bndf (left) and Osmr (right) expression in each major cell type in the four treatment groups. Osmr expression is used as a marker of reactive astrocytes. (B) Bar graph showing quantification of Bdnf + astrocytes (Bdnf+/Osmr+) in normothermic and hypothermic TBI cortex. n = 3 biological replicates per group. *p < 0.05, Student’s unpaired t-test. (C) RNAscope in situ hybridization to detect Osmr (red) and Bdnf (green) in hypothermic (left) and normothermic (right) TBI cortex. Cell nuclei labeled with DAPI (blue). Scale bar = 300 μm. (D) High magnification images of the injured cortex showing colabeling of Osmr and Bdnf. Scale bar = 15 μm