Fig. 6
From: Single cell RNA sequencing after moderate traumatic brain injury: effects of therapeutic hypothermia
Astrocytes are the most transcriptionally responsive to therapeutic hypothermia and upregulate BDNF. (A) Volcano plot of DEGs between hypothermia treated reactive astrocytes and normothermic reactive astrocytes. Top DEGs are labeled in red. (B) Quantification of the number of TBI-induced DEGs comparing normothermia and hypothermia for each major cell type. (C) UMAP feature plot of Bdnf expression across treatment groups. (D) Ingenuity Pathway Analysis of reactive astrocytes shows pathways that are affected by therapeutic hypothermia. Bars represent the -log(p-value) and color represents Z-scores; blue denotes negative Z-scores indicative of inhibition, and orange bars denote pathways with positive Z-scores indicative of activation. The threshold line indicates p < 0.05