Fig. 3

Heterogeneity of neural cells after moderate TBI. (A) UMAP of neural cells combined from sham and TBI normothermic animals (n = 3 biological replicates per group). Cells are colored by neural cell subtype. (B) Same UMAP arranged by injury condition, sham (red) and TBI (yellow), shows TBI-specific cell types. (C) Dot plot of unique marker genes used to identify each major cell type. (D) Proportion of astrocyte subtypes before and after TBI shows cell type-specific effects of TBI. Each data point is a biological replicate. Error bar = SEM. (E) Volcano plot showing DEGs between GFAP-hi astrocytes (positive fold change) and reactive astrocytes (negative fold change). (F-G) GO biological processes based on the top DEG comparing reactive astrocytes (F) and Gfap-hi astrocytes (G) and plotted on a log scale of its p-value (p < 0.001). The size of the circle represents the number of genes that defines the GO term, and the color of the circle represents its odds ratio. (H) Ingenuity Pathway Analysis on the top DEGs between reactive astrocytes and sham astrocytes (Gfap-low and Gfap-hi). Pathways with p < 0.05 (threshold line on graph) shown