Fig. 1

EED226 ameliorates symptoms and inflammation in EAE mice. A Scheme for the induction, assessment and drug administration of EAE mice. B Clinical scores of vehicle or EED226 treated EAE mice. n = 10 animals, 5 animals are sacrificed for the histopathological examination and flow cytometry analysis at day 22 post immunization (dpi 22). * indicates a statistically difference when compared with EAE + vehicle group. C Representative images of H&E staining (top) and LFB staining (bottom) for spinal cords from vehicle or EED226 treated EAE mice at dpi 22. Scale bar: 50 μm. n = 3. D Bar graph for LFB staining analysis of the demyelinated area in the spinal cords of vehicle or EED226 treated EAE mice at dpi 22. n = 3. E Representative images (left) and bar graph (right) for electron microscopy analysis of the demyelination in the spinal cords of vehicle or EED226 treated EAE mice at dpi 22. Scale bar: 2 μm. n = 3. F Representative images (left) and bar graph (right) for flow cytometry analysis of the frequency of naïve T cells (CD4⁺CD62L^hiCD44^lo) and central memory T cells (CD4⁺CD62L^loCD44^hi) in the spleen of vehicle or EED226 treated EAE mice at dpi 22. n = 3. G Representative images (left) and bar graph (right) for flow cytometry analysis of the frequency of Th1 (CD4⁺IFN-γ⁺) and Th17 (CD4⁺IL-17⁺) in the spleen of vehicle or EED226 treated EAE mice at dpi 22. n = 3. H Representative images (left) and bar graph (right) for flow cytometry analysis of the frequency of total DCs (CD11c⁺) in the spleen of vehicle or EED226 treated EAE mice at dpi 22. n = 3. I Representative images (left) and bar graph (right) for flow cytometry analysis of the frequency of mature DCs (CD11c⁺CD80⁺, CD11c⁺MHC-II⁺) in the spleen of vehicle or EED226 treated EAE mice at dpi 22. n = 3. J Bar graph for flow cytometry analysis of the percentage of CD80⁺ or MHC-II⁺ in CD11c⁺ DCs in the spleen of vehicle or EED226 treated EAE mice at dpi 22. n = 3. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant