Fig. 6

STING mediates the therapeutic effect of ACT001 on EAE. (A-B) The severity of EAE in wild-type (WT) and Sting1^−/− mice receiving ACT001 treatments (n = 6 per group). (A) Mean daily disease score of symptomatic mice. (B) Cumulative clinical score of EAE symptomatic mice. (C-G) Infiltration of inflammatory cells in the SCs at the peak of EAE (n = 6 per group). (C) Quantitative analysis of inflammatory cell infiltration areas in transverse sections of the SCs tissue in (D). (D) Representative H&E staining pictures. (E) Representative flow cytometry analysis of SCs-infiltrating Th1 and Th17 cells. (F-H) Statistical analysis of the frequencies and absolute numbers of Th1 (F), IFNγ⁻ Th17 (G), IFNγ⁺ Th17 and cells (H). Statistics were calculated using one-way ANOVA with Tukey’s correction. Error bars represent the mean ± SEM with ns indicating not significant, *p < 0.05, **p < 0.01, and ***p < 0.001