Fig. 2

ACT001 dampens the effector program of Th17 in the spinal cords of EAE mice. (A-B) Flow cytometry analysis of CD4⁺ T cells in iLNs and SCs was performed at the peak of EAE (n = 6 per group). (A) Representative flow cytometry results of CD4⁺ T cell gated on CD45⁺ cells. (B) Statistical analysis of the frequencies and absolute numbers of CD4⁺ T cells in (A). (C-D) Flow cytometry analysis of Treg cells in iLNs and SCs at the peak of EAE. (C) Representative flow cytometry results of CD4⁺Foxp3⁺ Treg cells. (D) Statistical analysis of the frequencies and absolute numbers of Treg cells in (C). (E-H) Flow cytometry analysis of Th1 and Th17 cells in iLNs and SCs at the peak of EAE. Representative flow cytometry results are shown in (E), along with statistical analysis of the frequency and absolute numbers of Th1 (F), IFNγ⁻ Th17 (G), and IFNγ⁺ Th17 (H) cells. (I-J) Normalized mRNA expressions of cytokines (I) and transcription factors (J) associated with Treg, Th1 and Th17 in CD4⁺ T cells isolated from SCs at the peak of EAE. Statistics were calculated using the unpaired sample t-test. Error bars represent the mean ± SEM, with ns indicating not significant, *p < 0.05, and **p < 0.01