Fig. 4
Time course of functional and structural changes within the visual system in EAE-induced mice. A) Visual acuity measured over time by changes in cycles/degree shows significant decline starting at two weeks post induction in MOG35 − 55-induced EAE mice compared to control mice, which is representative of other published studies (Godwin et al. Biomolecules 2022, Khan et al. Scientific Reports 2019). B) In accordance to Cruz-Herranz et al. Journal of neuroinflammation 2019 and Manogaran et al. Acta Neuropathologica 2019, analysis of average RNFL thickness over time demonstrates significant swelling followed by thinning of the RNFL in MOG35 − 55-induced EAE mice. For better transparency, methods relevant to EAE-induction and ophthalmic measurements shown in Fig. 4A and B are available in the Additional File 1. C) Representative OCT images measuring the RNFL indicate swelling during the acute phase of MOG35 − 55 EAE-ON and thinning at the end stage timepoint. D) Average pERG waveforms of MOG35 − 55-induced EAE and control mice show significant decrease in P1 to N2 amplitude in MOG35 − 55-induced EAE mice 60 days post induction (Fig. 4C and D adapted from Elwood et al. TVST 2024)