Fig. 5

IFN-γ inhibits HCMV infection of human neurons. A The experimental system for B. SH-SY5Y cells were treated with recombinant IFN-γ. The following day, cells were infected with 10 MOI of KL7-EGFP HCMV and at 2 dpi EGFP fluorescence was recorded and expressed as an average 8-bit green channel pixel intensity for each well. B Quantification of EGFP fluorescence. The data are merged from two independent experiments, with three technical replicates in each experiment. Unpaired two-tailed Student’s test was used. C Experimental scheme for D. SH-SY5Y cells were infected with 10 MOI of KL7-EGP HCMV. One hour post-infection, cells were treated with recombinant IFN-γ and at 2 dpi EGFP fluorescence was recorded and expressed as an average 8-bit green channel pixel intensity for each well. D Quantification of EGFP fluorescence. The data are merged from two independent experiments, with three technical replicates in each experiment. Unpaired two-tailed Student’s test was used. E hfOBSCs were infected with KL7-EGFP HCMV. Fluorescent microscopy monitoring of GFP expression in the hfOBSC at 2 dpi F Schematic representation of human brain slice cultures either untreated or treated with IFN-γ, and subsequently infected with HCMV. G hfOBSCs were infected with KL7-EGFP HCMV. One group of brain slices was treated with 1000 U/ml of recombinant IFN-γ. Brain slices were collected 2 dpi. HCMV⁺ NeuN⁺ cells were quantified. Mean values ± SD are shown (n = 2 donors, 4 section/condition/donor). Unpaired two-tailed Student’s test was used. p values indicate statistically significant differences