Fig. 4

IFN-γ and IL-12 promote the resolution of productive MCMV infection in neurons. Newborn C57BL/6, A Prf1^−/−, B Gzma^−/−Gzmb^−/−, C TNFRp55^−/−, D-G IFN-γ^−/−, and H-K Il12rb2^−/− mice were infected with MCMV on PND1. A-D, H Viral titers in the brain were determined by plaque assay at 14, 30 and, 70 dpi (n = 5–14). Titers in organs of individual mice are shown (circles). Black horizontal lines indicate the median values. D.L., detection limit. Mann–Whitney two-tailed test was used. E-G, I-K Immunohistochemical staining of MCMV infection in neurons at day 37 p.i. in E-G IFN-γ^−/−, and I-K Il12rb2^−/− mice. E, I Localization of IE1 protein-positive cells in the murine brain. Median values are shown (n = 5, 10 sections/mouse). Other refers to IE1⁺ cells outside the cortex and hippocampus in the brain. Kruskal–Wallis test was used. F, J Representative images of MCMV infected MAP2⁺ neuron. Arrows point to the MCMV-infected neurons (40x magnification, 100x magnification insert). G, K Quantification MCMV infected MAP2⁺ neurons (n = 5 mice, G = 10 sections/mouse, K = 20 sections/mouse). Columns indicate median values. Mann–Whitney two-tailed test was used. p values indicate statistically significant differences