Febrile temperature-regulated TRPV1 in CD4+ T cells mediates neuroinflammation in complex febrile seizures

Kong, Shuo; Jia, Xianglei; Liang, Xin; Chen, Yu; Liang, Jingyi; Zhang, Yan; Wu, Ningyang; Su, Song; Chen, Taoxiang; He, Xiaohua; Yin, Jun; Han, Song; Liu, Wanhong; Fan, Yuanteng; Xu, Jian; Peng, Biwen

doi:10.1186/s12974-025-03421-7

Journal of Neuroinflammation

Table 2 qPCR primer sequences

From: Febrile temperature-regulated TRPV1 in CD4⁺ T cells mediates neuroinflammation in complex febrile seizures

Primer	Froward primer 5′-3′	Reverse primer 5′-3′
β-Actin	CACGATGGAGGGGCCGGACTCAT	TAAAGACCTCTATGCCAACACAGT
T-bet	ACTAAGCAAGGACGGCGAA	GGGGAGTCTGGGTGGACATA
GATA3	GGGGAGTCTGGGTGGACATA	GGATACCTCTGCACCGTAGC
FOXP3	CCCCTCTAGCAGTCCACTTCA	AAGTTGCCGGGAGAGCTGAAT
RORγt	CACGGCCCTGGTTCTCAT	CAGATGTTCCACTCTCCTCTTCTCT
STAT3	TACCATTGACCTGCCGATGT	TCAAACGTGAGCGACTCAAAC
GM-CSF	ACAACCTGGGGGAAGGCTC	GACTTCTACCTCTTCATTCAACGTG
IL-17A	CTCCAGAAGGCCCTCAGACTAC	GGGTCTTCATTGCGGTGG
IL-17F	CCCATGGGATTACAACATCACTC	CACTGGGCCTCAGCGATC
IL-1βR	GTGCTACTGGGGCTCATTTGT	GGAGTAAGAGGACACTTGCGAAT
IL-23R	GGAGTAAGAGGACACTTGCGAAT	TCAGTGCTACAATCTTCAGAGGACA
TGF-βR	TCTGCATTGCACTTATGCTGA	AAAGGGCGATCTAGTGATGGA
IL-10	ATAACTGCACCCACTTCCCAGTC	CCCAAGTAACCCTTAAAGTCCTGC
TRPV1	CCCAAGTAACCCTTAAAGTCCTGC	AATCTGTCCCACTTGTCCTGC
TRPV2	TGTGGCCAGTGGTCCTGAT	TGGCTGGGACCCCTCA
TRPV3	TTGGATTTGGAGTAGCGCTGG	CCAGGCCTATGGTGAGCTTG
TRPV4	TCAACTCGCCCTTCAGAGACA	AAGTAGCCTCCCTCATCCTTGG
NFAT1	CCAGATCACAGCACACGGT	CGATCGGTTCTTCTCGGTCC
NFAT2	CCCGGAGTTCGACTTCGATT	TAACTGTAGTGTTCTGCGGC
NFAT4	CTGTCAATGGAGGCCACGAA	CAAGGACAATGTGAGCCCCT
ZO-1	GGAGATGTTTATGCGGACGG	TCCATTGCTGTGCTCTTAGCG
Ocldn	GTCCTCCTGGCTCAGTTGAA	AGATAAGCGAACCTTGGCGG
Cldn5	GGTCTGGTGCTGTGTCTGGTA	ATACACCTTGCACTGCATGTG
IL-1β	GCCACCTTTTGACAGTGATGAG	ACGGGAAAGACACAGGTAGC
IL-6	GCCTTCTTGGGACTGATGCT	TGCCATTGCACAACTCTTTTC
iNOS	ACCAAAGTGACCTGAAAGAGGAA	CCAGGGATTCTGGAACATTCTGTG
MHC II	GATTTCTACCCAGGCAGCATT	GGTCATTTCCAGCATCACCAGGA
CD80	CCTCGCTTCTCTTGGTTGGA	GGAGGGTCTTCTGGGGGTTT
CD86	ATGGACCCCAGATGCACCA	TGTGCCCAAATAGTGCTCGT
CCL2	TGCCCTAAGGTCTTCAGCAC	AAGGCATCACAGTCCGAGTC
CCL3	GGGCATATGGCTTCAGACACC	CAGTCGGGGTGTCAGCTCCAT
CCL4	CTTCTGTGCTCCAGGGTTCTC	CTGCCTCTTTTGGTCAGGAATACCA
CCL5	AATCTTGCAGTCGTGTTTGTC	AGGGGATTACTGAGTGGCAT
CCL9	GCCCAGATCACACATGCAAC	AGGACAGGCAGCAATCTGAA
CXCL10	CCCACGTGTTGAGATCATTGCC	TCTGCTGTCCATCCATCGC
CXCL11	CCACGCTACCTTCTGTGGTT	ATGTTCGTGTGCCTCGTGAT
CXCL12	CACTTCTATGCCTCCTGCTCA	CAGTATGGTCCTGAAGATCACAGC
CCL18	CTCTGCTGCCTCGTCTATACC	GCCTCTCTTGGTTAGGAGGATGAC
CCL20	TCTGTGTGCGCTGATCCAAA	CAGTGATGTGCAGGTGAAGC

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ISSN: 1742-2094

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