Table 1. Antibodies applied in this study
| Â | Manufacture | Dilution |
|---|---|---|
Primary antibody | ||
Anti-CD4 | Abcam (ab133616) | 1:200 (IF) |
Anti-IL-17A | Abcam (ab318150) | 1:200 (IF) |
Anti-TRPV4 | Affinity (DF8624) | 1:500 (WB) 1:200 (IF) |
Anti-TRPV1 | Affinity (DF10320) | 1:500 (WB) 1:200 (IF) |
Anti-NFAT2 | Novus (NB300-504) | 1:200 (IF) |
Anti-NFAT4 | Affinity (AF6240) | 1:200 (IF) |
Anti-STAT3 | Proteintech (10253-2-AP) | 1:500 (WB) |
Anti-P-STAT3 | CST (9145) | 1:1000 (WB) 1:200 (IF) |
Anti-ZO-1 | Proteintech (21773-1-AP) | 1:1000 (WB) |
Anti-Ocldn | Proteintech (27260-1-AP) | 1:1000 (WB) |
Anti-Cldn5 | Affinity (AF5216) | 1:1000 (WB) |
Anti-IBA1 | CST (17198S) | 1:1000 (WB) 1:200 (IF) |
Anti-CD68 | Proteintech (28058-1-AP) | 1:1000 (WB) 1:200 (IF) |
Anti-P38 | Proteintech (14064-1-AP) | 1:1000 (WB) |
Anti-P-P38 | Proteintech (28796-1-AP) | 1:1000 (WB) |
Anti-IL-6 | Bioss (bs-0782R) | 1:500 (WB) |
Anti-TNF-α | ABclonal (A0277) | 1:500 (WB) |
Anti-β-actin | Proteintech (66009-1-Ig) | 1:5000 (WB) |
Anti-GAPDH | Proteintech (66004-1-Ig) | 1:5000 (WB) |
Secondary antibody | ||
Goat anti-mouse IgG | Biosharp(BL001A) | 1:10000 (WB) |
Goat anti-rabbit IgG | Biosharp(BL003A) | 1:10000 (WB) |