Fig. 6

Th17 cells activate microglia and exacerbate the inflammation via IL-17A following CFS. A Representative fluorescence images of cortical Th17 cells infiltration in WT and TRPV1^−/− mice after CFS and statistical analysis of Th17 cells numbers (n = 6 for each group, Data are presented as mean ± SEM, One-way ANOVA). B Relative protein analysis of microglial activation marker CD68/P-P38 and inflammatory cytokines IL-6/TNFα in cortical homogenates from WT and IL-17A^−/− mice with or without CFS; corresponding quantitative data (n = 3 for each group, Data are presented as mean ± SEM, One-way ANOVA). C Representative fluorescent images of activated microglia (IBA1⁺CD68⁺ cells) in cortical regions from WT and IL-17A^−/− mice with or without CFS; the number of IBA1⁺CD68⁺ cells/0.1mm² (n = 6 for each group, Data are presented as mean ± SEM, One-way ANOVA); quantification of microglial branch length (n = 12 for each group, Data are presented as mean ± SEM, One-way ANOVA). D The mRNA expression levels of microglial activation marker CD80/CD86/MHC II and inflammatory factors iNOS/ IL-6/IL-1β in cortical homogenates from WT and IL-17A^−/− mice with or without CFS (n = 4 for each group, Data are presented as mean ± SEM, One-way ANOVA)