Fig. 5

TRPV1^−/− or IL-17A^−/− alleviates the reduction of tight junction-associated proteins after CFS. A Scheme of in vivo experiments of TJs analysis in WT and TRPV1^−/− mice after CFS or not. B mRNA expression levels of TJs (ZO-1/Ocldn/Cldn5) (n = 4 for each group, Data are presented as mean ± SEM, One-way ANOVA). C SEM data showing TJs between endothelial cells; arrows: tight junctions between two endothelial cells. D Representative protein bands of TJs (ZO-1/Ocldn/Cldn5) in cortical brain homogenates and corresponding statistical analysis (n = 4 for each group, Data are presented as mean ± SEM, One-way ANOVA). E Scheme of in vivo experiments of TJs analysis in the cortical region in WT and IL-17A^−/− mice with or without CFS. F mRNA expression levels of TJs (ZO-1/Ocldn/Cldn5) (n = 6 for each group, Data are presented as mean ± SEM, One-way ANOVA). G SEM data showing disrupted tight junctions between endothelial cells; arrows indicate tight junctions between two endothelial cells. H Representative protein bands of TJs (ZO-1/Ocldn/Cldn5) in WT and IL-17A^−/− mice after CFS or not, and corresponding statistical analysis (n = 3 for each group, Data are presented as mean ± SEM, One-way ANOVA)