Fig. 3

TRPV channels expression was upregulated in CD4⁺ T cells under hyperpyrexia. A Schematic of detecting the expression TRPV channels in naïve CD4⁺ T cells cultured at 37 ℃ or 39.5 ℃ for 3 days. B The qPCR analysis showing the mRNA level of TRPV1-4 after 3 day’s culture under 37 ℃ or 39.5 ℃ (n = 4 for each group, Data are presented as mean ± SEM, Student’s t test). C Heat map of genome-wide genes (top15, upregulated in 39.5 ℃) in CD4⁺ T cells cultured at 37 ℃ or 39.5 ℃ (n = 3 for each group). D Representative immunoblot bands and the bands analysis of TRPV1/4 in CD4⁺ T cells cultured at 37 ℃ or 39.5 ℃ (n = 4 for each group, Data are presented as mean ± SEM, Student’s t test). E Representative confocal immunofluorescent images of TRPV1/4 in CD4⁺ T cells cultured at 37 ℃ or 39.5 ℃ and the analysis of fluorescence intensity (n = 6 for each group, Data are presented as mean ± SEM, Student’s t test)