Fig. 2

Th17 cells involvement in seizure susceptibility following CFS. A Representative confocal images showing CD4⁺IL-17A⁺ cells (CD4: green IL-17A: red) in cortical from the mice of Ctrl or CFS group and the total number of CD4⁺IL-17A⁺ cells (Ctrl group: n = 6 CFS group: n = 7, Data are presented as mean ± SEM, Student’s t test). B Flow cytometry analysis was used to measure the counts of Th17 cells. left: Th17 cell from blood of Ctrl or CFS group. right: Th17 cell from spleen of Ctrl or CFS group (Ctrl group: n = 4 CFS group: n = 5, Data are presented as mean ± SEM, Student’s t test). C IL-17A in the serum of Ctrl group (n = 4) and CFS group (n = 5) measured by ELISA. Data are presented as mean ± SEM, Student’s t test. D The qPCR analysis showing mRNA levels of key transcription factor genes of Th1(T-bet), Th2(GATA3), Treg (FOXP3) and Th17(STAT3/RORγt); Th17 key cytokine genes, GM-CSF, IL-17A, IL-17F and IL-10; Th17 key receptor genes, IL-1βR and IL-23R (n = 4 for each group, Data are presented as mean ± SEM, Student’s t test)