Fig. 1

Mouse models exhibit increased susceptibility to epilepsy following CFS. A Schematic diagram of CFS model induction and the process for evaluating seizure susceptibility (top); seizure latency (middle) and seizure severity (bottom) (n = 5, Data are presented as mean ± SEM, Kolmogorov–Smirnov test). B Gene Ontology (GO) pathway analysis results comparing Ctrl group and CFS group (Ctrl n = 6, CFS n = 6). C Representative MEA voltage recordings from a mouse cortical slice exhibiting spontaneous action potentials firing, shown as spikes on the trace in artificial cerebrospinal fluid (ACSF)(left); the presence of 4-AP (5 mM) (right); the time calibration line in the Raster plot is 20 s. A representative trace recorded by MEA electrode, when the perfusion with 5 mM 4-AP, epileptiform activity was evoked. Top row shows voltage recording from Ctrl group and bottom row is from CFS group. The number of action potentials recorded by all electrodes within 0.1 s from Ctrl group (n = 7) or CFS group (n = 7). Data are presented as mean ± SEM. Student’s t test