Fig. 3

The impact of TREM2 expression on neurodegeneration

NeuN immunofluorescence staining was carried out on PS19 mice expressing either TREM2-WT or TREM2-R47H. (A-B) Representative images (A) and quantification (B and C) of the lateral ventricle area in mice following the induction of TREM2-WT or TREM2-R47H expression in microglia within the PS19 background. Scale bar, 1 mm. (D-F) Representative images (D) and quantification (E and F) of the thickness of the pyramidal neuronal layer in the piriform cortex in mice upon induction of TREM2-WT or TREM2-R47H expression in microglia within the PS19 background. Scale bar, 500 μm. (G-K) Representative images (G) and quantification (H-K) of the thickness of the neuronal layers in the in mice following induction of TREM2-WT or TREM2-R47H expression in microglia within the PS19 background. Scale bar, 500 μm. Sample size: n = 19–23 mice per group, with mixed sexes. Each datapoint (circle) represents an individual animal, male and female mice are labeled as solid and open circles, respectively. Data are presented as mean ± SEM. Statistical analysis was conducted using Mann–Whitney tests. P values less than 0.05 were considered statistically significant. *P < 0.05; N.S., not significant.