Fig. 2

The impact of TREM2 expression on tau pathology

(A-C) Representative images of piriform cortical region (A) and quantification (B and C) of p-tau by immunofluorescence staining with AT8 antibody. Scale bar, 500 μm. (D-F) Representative images of hippocampus (D) and quantification (E and F) of p-Tau by AT8 immunofluorescence staining. Scale bar, 500 μm. (G-R) The levels of p-tau and total tau in the buffer soluble (RAB), detergent soluble (RIPA), and insoluble (FA) fractions were detected by ELISAs in the mice with or without TREM2-WT or TREM2-R47H overexpression. Sample size: n = 19–23 mice/group, with mixed sexes. Each datapoint (circle) represents an individual animal, male and female mice are labeled as solid and open circles, respectively. Data are shown as mean ± SEM. Mann–Whitney tests were used for statistical analysis. P values < 0.05 were considered to be statistically significant. **P < 0.01; N.S., not significant.