Fig. 2

Moss-derived recombinant human CFH variants attenuate mononuclear phagocyte reactivity in light-exposed retinas. A Representative images from the outer plexiform layer (OPL) of Iba1-stained retinal flat mounts from control and light-exposed mice one, three and four days after light exposure. Scale bar: 100 µm. B Pro-inflammatory cytokine and complement levels in the retina of control and light-exposed mice at indicated time point. C Representative Western blot of C3d positive C3 cleavage products 1d after light exposure. D Quantification of C3 cleavage products normalized to actin band intensity and no light control. Data shown as mean ± SEM, n = 3–9 retinas. One-way ANOVA followed by Sidak’s multiple comparison test; *P < 0.05, **P < 0.01 and ***P ≤ 0.001. N.d. not detected