Fig. 2
Asrij depletion reduces Aβ plaque deposition and associated pathology in APP/PS1 mice. A Representative confocal images of brain sections showing Thioflavin S (ThioS) staining (green) for plaques. DAPI (blue) marks the nuclei. Graphs show quantification of percent area covered by ThioS+ staining in hippocampus and cortex (n = 6, all males). B Representative confocal images of brain sections showing Aβ staining (red) using D54D2 antibody. DAPI (blue) marks the nuclei. Graphs show quantification of percent area covered by Aβ staining in hippocampus and cortex (n = 5, all males). C Graphs show quantification of Aβ42 levels (pg/mL) in the soluble (PBS) and insoluble (guanidine) brain fractions by ELISA (n = 6, all males). D Representative confocal images showing S100B (magenta) and GFAP (red) staining for astrocytes in the cortex. ThioS (green) and DAPI (blue) mark plaques and nuclei, respectively. Graph shows quantification of GFAP+ astrocytes per field of view (FOV) of the cortex averaged over 4 FOVs per section with 3 sections per mouse (n = 3 for WT and KO, n = 6 for AD and AD/KO, all males). E Representative confocal images showing LAMP1 staining (magenta) around ThioS+ Aβ plaques (green). Graph shows quantification of LAMP1+ area per plaque averaged over 15 plaques per section with 3 sections per mouse (n = 5, all males). F Representative confocal images showing APP staining (green) around 6E10+ Aβ plaques (red). Graph shows quantification of APP+ area per plaque averaged over 15 plaques per section with 3 sections per mouse (n = 3, all males). G Immunoblot analysis of PSD95 and Synaptophysin levels in the hippocampus. α-tubulin is loading control. Graphs show fold change in PSD95 and Synaptophysin levels normalized to α-tubulin (n = 6, all males). Statistical significance between experimental groups was calculated by unpaired two-tailed Student’s t-test (A-C and E-F) and one-way ANOVA with Tukey’s post hoc test (D and G). Error bars denote mean ± SEM. ns: non-significant, *P < 0.05, **P < 0.01, and ***P < 0.001