Fig. 7

STAT6 is a common transcription factor of Mertk and Cd36 and is dephosphorylated by SHP1 following SAH. A-C. Potential STAT6-binding site in the promoter region of the Mertk and Cd36. D. Expression of Stat6 in microglia from RNA-seq results (n = 3 each group). E. Western blot assay for the expression of p-STAT6 in microglia following mutation of the intracellular domain of SIRPα. F. Quantification of p-STAT6 protein levels (n = 3 each group). G. Western blot assay for the expression of p-STAT6 and STAT6 in primary microglia after 10µM Hb and ACs stimulation. H-J. Quantification of p-STAT6, STAT6 and p-STAT6/STAT6 protein levels (n = 3 each group). K-M. Representative agarose gel electrophoresis images and quantification for PCR products of ChIP assay (n = 3 each group). N-O. Quantification of luciferase activity of MerTK and CD36 in Ctrl, MerTK_mut and CD36_mut microglia with or without STAT6-knockdown (n = 3 each group). P. Association between STAT6 and SHP-1 was assessed by Western blot analysis after the Co-IP of STAT6 in Ctrl and SIRPα_mut microglia after 10µM Hb and ACs stimulation. Q. Quantification of p-SHP1 and p-STAT6 protein levels interacted with STAT6 (n = 3 each group). All values are means ± SEM, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns, no significant changes