Fig. 6

Mutation of the intracellular domain of SIRPα enhances the expression of MerTK and CD36. (A) The Venn diagram illustrates that 11,025 genes are expressed in both groups. (B) Volcano plot of differentially expressed genes (DEGs) in microglia following mutation of the intracellular domain of SIRPα. X-axis: log2 ratio of differentially expressed genes between SIRPα_mut and Ctrl. Y-axis: the p-value (-log10 transformed) of proteins, fulfilling the criteria|log2 (fold change)| ≥ 1 and P < 0.05 (n = 7 each group). (C) GO enrichment analysis of DEGs between SIRPα_mut and Ctrl, fulfilling the criteria|log2 (fold change)| ≥ 1 and P < 0.05 (n = 7 each group). (D) Heatmap analysis of co-expressed genes in DEGs and the top 100 phagocytosis-related genes identified by Genecards. (E) Heatmap analysis of co-expressed genes in DEGs and the top 100 efferocytosis-related genes identified by Genecards. F-G. Expression of Mertk and Cd36 in microglia from RNA-seq results (n = 3 each group). H. Western blot assay for the expression of MerTK and CD36 in microglia following mutation of the intracellular domain of SIRPα. I-J. Quantification of MerTK and CD36 protein levels (n = 3 each group). K. Western blot assay for the expression of MerTK and CD36 in primary microglia after 10µM Hb and ACs stimulation. L-M. Quantification of MerTK and CD36 protein levels (n = 3 each group). All values are means ± SEM, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns, no significant changes