Fig. 5

Mutation of the intracellular domain of SIRPα enhances efferocytosis by microglia and mitigates neuroinflammation following SAH in vitro A-B. Flow cytometry analysis and quantification of CMFDA⁺Iba1⁺ microglia (n = 3 each group). C. Representative immunofluorescence images of CMFDA (green) and Iba1 (red) in microglia after 10µM Hb and ACs stimulation for 12 h. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. D. Quantification of CMFDA⁺Iba1⁺ microglia numbers (n = 3 each group). E. Representative immunofluorescence images of Lysosensor (green) in microglia after 10µM Hb and ACs stimulation for 12 h. Live cells were counterstained with Hoechst (blue). Scale bar, 50 μm. F. Quantification of fluorescence intensity of Lysosensor (n = 3 each group). G. The levels of IL-1β, IL-18, IL-10 and TGF-β1 in culture medium from the co-culture system (n = 3 each group). H. Representative images of MAP2-stained neurons after co-cultured with microglia. Scale bar, 50 μm. I. Quantification of the number of MAP2⁺ (red) neurons (n = 3 each group). All values are means ± SEM, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns, no significant changes