Fig. 4

SHP1 and SHP2 recruitment and phosphorylation by the intracellular domain of SIRPα in regulating efferocytosis following SAH in vitro. A. Association between SIRPα, SHP-1, and SHP-2 was assessed by Western blot analysis after the co-immunoprecipitation (Co-IP) of SIRPα in microglia after 10µM Hb and ACs stimulation. B-D. Quantification of SIRPα, and p-SHP1, p-SHP2 protein levels interacted with SIRPα (n = 3 each group). E-F. Quantification of p-SHP1/SHP1 and p-SHP2/SHP2 protein levels (n = 3 each group). G. Association between SIRPα and SHP-1 was assessed by Western blot analysis after the Co-IP of SIRPα in Ctrl and SIRPα_mut microglia after 10µM Hb and ACs stimulation. H. Quantification of p-SHP1 protein levels interacted with SIRPα (n = 3 each group). All values are means ± SEM, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns, no significant changes