Fig. 1

SIRPα levels are elevated in CSF of patients with SAH and associated with prognosis. (A) Schematic diagram of lumbar puncture for CSF collection and proteomic analysis. (B) Volcano plot of upregulated proteins and downregulated proteins in CSF of patients with SAH. X-axis: log2 ratio of protein levels between CSF of patients with SAH and healthy control. Y-axis: the p-value (-log10 transformed) of proteins, fulfilling the criteria|log2 (fold change)| ≥ 1 and P < 0.05 (n = 7 each group). (C) KEGG enrichment analysis of differential protein levels between CSF of patients with SAH and healthy control, fulfilling the criteria|log2 (fold change)| ≥ 1 and P < 0.05 (n = 7 each group). (D) Heatmap representing differential protein levels in the “Regulation of Inflammatory Response” pathway from GO enrichment analysis, fulfilling the criteria|log2 (fold change)| ≥ 1 and P < 0.05 (n = 7 each group). (E) LFQ intensity of SIRPα protein levels in the CSF of SAH patients and healthy controls (n = 7 each group). (F) IL-18 levels in the CSF of SAH patients compared to healthy controls (n = 8 each group). (G) IL-1β levels in the CSF of SAH patients compared to healthy controls (n = 7 each group). (H) SIRPα levels in the CSF of SAH patients compared to healthy controls (n = 9 each group). (I) Simple linear regression analysis between SIRPα and IL-1β levels in SAH patients (n = 14 each group). (J) Simple linear regression analysis between SIRPα and IL-18 levels in SAH patients (n = 14 each group). K. Simple linear regression analysis between SIRPα levels and Hunt-Hess grade in SAH patients (n = 14 each group). All values are means ± SEM, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns, no significant changes