Fig. 3
From: MAIT cell deficiency exacerbates neuroinflammation in P301S human tau transgenic mice
MAIT cells protect meningeal barrier integrity and repress proinflammatory cytokine expression from microglia. A Brain vibratome sections were obtained from 7-month-old Mr1−/− P301S mice and control Mr1+/+ P301S mice with transcranial administration of SR101. Representative imaging of sections at 300 µM to 600 µM lateral to bregma. B Quantification of fluorescence intensity of SR101 at 50 µM below the leptomeningeal cells in the brain vibratome sections of 7-month-old Mr1−/− P301S mice and control Mr1+/+ P301S mice. C Immunofluorescence staining of IBA1 in hippocampus DG of 7-month Mr1−/− P301S mice and control Mr1+/+ P301S mice. D Percentages of IBA1 + areas in the hippocampus DG of 7-month Mr1−/− P301S mice and control Mr1+/+ P301S mice. E Representative flow cytometry profile showing gating strategy of microglia in the hippocampus. F Representative flow cytometry profiles showing expression of TNF in microglia in the hippocampus of 7-month-old Mr1−/− P301S mice and control Mr1+/+ P301S mice. G Mean fluorescence intensity of TNF in microglia in the hippocampus of 7-month-old Mr1−/− P301S mice and control Mr1+/+ P301S mice. H Representative flow cytometry profiles showing expression of the indicated molecules by hippocampus microglia in 7-month-old Mr1−/− P301S mice and control Mr1+/+ P301S mice. I Mean fluorescence intensity of the indicated molecules in microglia in the hippocampus of 7-month-old Mr1−/− P301S mice and control Mr1+/+ P301S mice. J MAIT cells were transferred to 6-week-old Mr1−/− P301S mice. Bain vibratome sections were obtained in mice with transcranial administration of SR101, when mice were 7-month-old. Representative images for mice with adoptive transfer of MAIT cells or PBS control. K Quantification of fluorescence intensity of SR101 at 50 µM below the leptomeningeal cells in mice with adoptive transfer of MAIT cells or PBS control. L Immunofluorescence staining of IBA1 in the hippocampus DG of 7-month-old Mr1−/− P301S mice with adoptive transfer of MAIT cells or PBS. M Percentages of IBA1 + areas in the hippocampus of 7-month-old Mr1−/− P301S mice with adoptive transfer of MAIT cells or PBS. N Representative flow cytometry profiles showing expression of TNF in microglia in the hippocampus of 7-month-old Mr1−/− P301S mice with adoptive transfer of MAIT cells or PBS. O Mean fluorescence intensity of TNF in the hippocampus of 7-month Mr1−/− P301S mice with adoptive transfer of MAIT cells or PBS. Data are from 4–6 mice per group, representative of 2 independent experiments. Error bars = mean ± SEM. **p < 0.01, *p < 0.05. Sex information in Additional file 1