Fig. 9

SPAK Inhibition by ZT-1a Reduces CNS Infiltration of CD4⁺ T Cell Subsets. A. Representative FCM plots showing CD4⁺, Th1⁺, and Th17⁺ T cell populations in CSF at day 14 post-EAE induction in vehicle- and ZT-1a-treated mice. B-D. Quantitative analysis of CSF FCM data demonstrating reduced infiltration of CD4⁺ (B), Th1⁺ (C), and Th17⁺ (D) T cells following ZT-1a treatment (n = 5/group). E. FCM analysis of CD4⁺, Th1⁺, and Th17⁺ T cell populations in spinal cords at day 14 post-EAE induction in vehicle- and ZT-1a-treated mice. F-H. Quantitative analysis of spinal cords FCM data demonstrating reduced infiltration of CD4⁺ (F), Th1⁺ (G), and Th17⁺ (H) T cells following ZT-1a treatment (n = 5/group). Statistical analyses performed using unpaired t-test. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. ChP: choroid plexus