Fig. 5

ErbB4 small molecule agonist enhances the ErbB4 signaling pathway in hippocampus of mice exposed to PS-MPs. (A) Immunofluorescence was used to detect levels of phosphorylated ErbB4 (p-ErbB4) and β3-tubulin. (B) Protein levels associated with the ErbB4 signaling pathway in the hippocampus of mice were assessed through western blot analysis. (C) A statistical analysis was performed on the data derived from protein levels, employing one-way ANOVA with Tukey’s multiple comparison tests. The p-ErbB4 is represented in red, while β3-tubulin is depicted in green, with a scale bar of 20 μm. The results are expressed as means ± SEM, with significance levels indicated by *p < 0.05, **p < 0.01, and ***p < 0.001. Each experimental group comprised n = 4 samples