Fig. 2

Dopamine receptor expression levels in primary human myeloid cells change with maturation. Primary human peripheral blood mononuclear cells (PBMCs), monocytes, and monocyte-derived macrophages (hMDM) were collected for subsequent mRNA analysis. 19 of these donors were paired across the three cell types. A qPCR detected mRNA for all subtypes of dopamine receptors (DRD1, DRD2, DRD3, DRD4 and DRD5) that were differentially expressed in these cell types (N = 33–42). B Using a D1-like to D2-like ratio (DRD1 + DRD5 / (DRD2 + DRD3 + DRD4), hMDM had a significantly higher ratio compared to monocytes or PBMCs. Significance was determined using Kruskal–Wallis tests and post-hocs with Dunn’s multiple comparisons,*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001