Fig. 5

Microglia reduction decreases CNV number in Vldlr^−/−Cx3cr1^CreERCsf1r^iDTR mice. A: Schematic for injection strategy in Vldlr^−/−Cx3cr1^CreERCsf1r^iDTR mice. B-C: Representative choroidal wholemount images of PBS- and DT-treated Vldlr^−/−Cx3cr1^CreERCsf1r^iDTR mice stained for IBA1 (macrophages) and Podocalyxin (choriocapillaris). Insets are outlined in yellow. DT (diphtheria toxin) treatment reduced macrophage counts (D, Mann-Whitney test) and decreased the number of CNV lesions (E, Student’s unpaired t-test). DT treatment but had no effect upon total CNV lesion area (F, Student’s unpaired t-test). **** p < 0.0001, * p < 0.05, ns = not significant. N = 33–35 mice per group