Fig. 4

Microglia depletion slightly decreases CNV number in Vldlr^−/−Tmem119^CreER/+Rosa^DTR/+ mice. A: Schematic for injection strategy in Vldlr^−/−Tmem119^CreER/+Rosa^DTR/+ mice. B-C: Representative choroidal wholemount images of PBS- and DT-treated Vldlr^−/−Tmem119^CreER/+Rosa^DTR/+ mice stained for IBA1 (macrophages) and Podocalyxin (choriocapillaris). Insets are outlined in yellow. DT (diphtheria toxin) treatment reduced macrophage counts (D, Student’s unpaired t-test) and decreased the number of CNV lesions (E, Welch’s t-test). DT treatment but had no effect upon total CNV lesion area (F, Student’s unpaired t-test). * p < 0.05, ns = not significant. N = 35–37 mice per group