Table 2 The immunity-related animal models of CIndU
Animals | Type | Drugs | Methods | Manifestations | References |
|---|---|---|---|---|---|
Female BALB/c mice | Contact urticaria | TMA | Mice were sensitized by applying 100 µL of TMA (500 mg/mL) in acetone/olive oil (4:1, v/v) to the shaved hind flank. Secondary sensitizations with 50 µL of TMA (250 mg/mL) were performed on days 7 and 10. On day 13, contact urticaria was induced by applying 25 µL of TMA (100 mg/mL) to the ears. | Ear swelling, itching and lesions on the skin | [70] |
Female Balb/c mice | Contact urticaria | TMA | A approximately 4 cm² area on the trunk of animals was shaved and tape-stripped three times before applying 100 µL of 500 mg/mL TMA topically. Allergy induction was repeated at the same site with 50 µL of 250 mg/mL TMA after 7 days. | Ear swelling | [71] |
BALB/c mice | ICU | TMA | ICU mice were depilated (2 cm × 3 cm) and treated with 100 µL TMA (500 mg/mL), followed by 50 µL TMA (250 mg/mL) daily for 7 days. On day 6, 25 µL TMA (100 mg/mL) was applied to both ears to induce ICU. | Wheals, scratching behavior, swelling | [72] |
Male C57BL/6 mice and BALB/c mice | Contact urticaria | DNP-IgE monoclonal antibody combined with DNFB | Model mice were injected with 0.2 ml of saline-diluted anti-DNP IgE antibody. After 24 h, 0.2% DNFB (Olive oil: Acetone = 1:3) was applied to both ears and the back of the mice, with the normal group receiving solvent. | Scratching behavior, ear swelling, and wheal | [39] |