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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Inhibition of neutrophil extracellular traps alleviates blood–brain barrier disruption and cognitive dysfunction via Wnt3/β-catenin/TCF4 signaling in sepsis-associated encephalopathy

Fig. 4

The inhibition of NETs formation reduced BBB permeability in SAE mice by alleviating TJs disruption. A The BWC was measured. Quantification of the FS extravasation in the hippocampus (B) and the global brain tissue (C) (n = 6). D Representative images showing the extravasation of FS. E Expression levels of TJs (ZO-1, Occludin, Claudin-5) proteins in the hippocampus were detected by Western blot (n = 6). F Expression levels of TJ proteins were quantified and normalized to GAPDH levels (n = 6). Double labeling shows colocalization of Occludin (G) and ZO-1 (I) with ECs (CD31) in the CA1 region of the hippocampus (40x, n = 6). Quantification of the fluorescence intensity of Occludin (H) and ZO-1 (J) in the hippocampus. NETs: Neutrophil extracellular traps; CLP: Cecal Ligation and Puncture; SAE: Sepsis-associated encephalopathy; ECs: endothelial cells; BBB: Blood–brain barrier; TJs: Tight junctions; BWC: Brain water content; FS: Fluorescein sodium. (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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Journal of Neuroinflammation

ISSN: 1742-2094