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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Inhibition of neutrophil extracellular traps alleviates blood–brain barrier disruption and cognitive dysfunction via Wnt3/β-catenin/TCF4 signaling in sepsis-associated encephalopathy

Fig. 2

The inhibition of NETs formation improved SAE mice survival, defended neuroinflammation and alleviated cognitive impairment. A 7-day survival rates of SAE mice treated with different concentrations of GSK484 (n = 15). B Quantification of NETs numbers (n = 6). C and D GSK484 inhibited NETs formation in the CA1 region of the hippocampus by IF staining (n = 6). E–H ELISA test showed that GSK484 decreased the levels of TNF-α, IL-6, IL-1β and IL-10 in hippocampus tissue (n = 6). I The trace heat map of OFT. The time (J) and the movement distance (K) in center in OFT. L The trace heat map of MWM test. The movement distance (M) in island, and the time (N) in NW area in the MWM test were increased after GSK484 treatment (n = 6). NETs: Neutrophil extracellular traps; CLP: Cecal Ligation and Puncture; SAE: Sepsis-associated encephalopathy; IF: immunofluorescence; OFT: open field test; MWM: Morris water maze. (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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Journal of Neuroinflammation

ISSN: 1742-2094