Fig. 1

Dynamic expression of neutrophils and NETs after CLP over a period of time. A Schematic timeline of the experimental procedures. B and C Representative photographs of IF co-staining of Ly-6G and CD11b, a neutrophil biomarker, and quantification of the number of Ly-6G/CD11b + cell in the hippocampal CA1 region of mice from the sham group and CLP group at 4 h, 8 h, 12 h, 24 h, 48 h, 72 h, 5 days and 7 days (n = 6). D and E Representative photographs of IF staining of Cit-H3 with MPO, a NETs biomarker, and quantification of the number of Cit-H3/MPO/DAPI + cells in the hippocampal CA1 region of mice from the sham group and CLP group at 4 h, 8 h, 12 h, 24 h, 48 h, 72 h, 5 days and 7 days (n = 6). Nuclei were stained with DAPI. Scale bar = 20 µm. NETs: Neutrophil extracellular traps, CLP: Cecal Ligation and Puncture; Cit-H3: Citrullinated histones H3; MPO: Myeloperoxidase; IF: immunofluorescence. (Data are expressed as the mean ± SD. *p < 0.05 compared with sham, ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)