Fig. 6
From: Integration and functionality of human iPSC-derived microglia in a chimeric mouse retinal model
Function of long-lived xMG in chimeric mouse retinal explants. (A) Representative images showing xMG distribution in mouse retina explants at non-injury and the injury site. xMG aggregate around the injury site. Scale bars, 50 μm. (B) Representative images showing phagosome labeled by anti-CD68 (light blue) in xMG with anti-Iba1 (purple) outlining cell morphology at 14 days post-transplantation. Left panels display fluorescent images, while the right panels depict 3D-surface images derived from the raw images. (C) Representative images showing PSD95-positive puncta engrafted in EGFP+ xMG with anti-Iba1 (yellow) outlining cell morphology in a mouse retinal explant at 14 days post-transplantation. The left panels show fluorescent images (arrows indicated engrafted puncta), while the right panels depict 3D-surface images derived from the raw images. Scale bars, 10 μm